Two persons, yet to be identified, have died at Yabram, a farming community in the Krachi East Municipality of the Oti Region.
The incident occurred on the Asukawkaw-Dambai highway on Tuesday at about 1550 hours.
The two motorbikes, travelling from opposite sides, collided head-on, leaving one female in critical condition.
She was subsequently rushed to Dambai Health Centre for emergency medical care.
Corporal Zakaria Asharift of the Motor Traffic and Transport Division told the Ghana News Agency that investigations were underway to ascertain what caused the accident.
He said the deceased has been taken to WoraWora Government Hospital.
He also urged the public to help the police to identify the deceased and appealed to commercial drivers to take extra care on the highway.